Human Somatostatin R5/SSTR5 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4448R-100UG
Detection of  Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with  Human SSTR5 and eGFP by Flow Cytometry.
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Human Somatostatin R5/SSTR5 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Somatostatin R5/SSTR5 in direct ELISAs. Stains Somatostatin R5/SSTR5 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 394401
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Somatostatin R5/SSTR5
Met1-Leu364
Accession # AAK61266
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of  Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with  Human SSTR5 and eGFP by Flow Cytometry. View Larger

Detection of Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with Human SSTR5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Somatostatin R5/SSTR5 or (B) irrelevant transfectants and eGFP was stained with Alexa Fluor® 647‑conjugated Mouse Anti-Human Somatostatin R5/SSTR5 Monoclonal Antibody (Catalog # IC4448R). Quadrant markers were set based on control antibody staining (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Somatostatin R5/SSTR5

Somatostatin Receptor type 5 (SSTR5) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Within the extracellular portions, human SSTR5 shares 65% and 68% aa identity with mouse and rat SSTR5, respectively. It is expressed in adult pituitary, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. It is an inhibitory receptor that transduces the antiproliferative and antisecretive effects of somatostatins.

Long Name
Somatostatin Receptor Type 5
Entrez Gene IDs
6755 (Human); 20609 (Mouse); 25354 (Rat)
Alternate Names
Somatostatin R5; somatostatin receptor 5; somatostatin receptor subtype 5; somatostatin receptor type 5; SomatostatinR5; SS5R; SS-5-R; SS5-R; SST5; SSTR5

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