Human Somatostatin R5/SSTR5 Antibody

Catalog # Availability Size / Price Qty
MAB4448
MAB4448-SP
Detection of Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with Human SSTR5 and eGFP by Flow Cytometry.
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Human Somatostatin R5/SSTR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Somatostatin R5/SSTR5 in direct ELISAs. Stains Somatostatin R5/SSTR5 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 394401
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Somatostatin R5/SSTR5
Met1-Leu364
Accession # AAK61266
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Somatostatin R5/SSTR5 antibody in HEK293 Human Cell Line Transfected with Human SSTR5 and eGFP antibody by Flow Cytometry. View Larger

Detection of Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with Human SSTR5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Somatostatin R5/SSTR5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Somatostatin R5/SSTR5 Monoclonal Antibody (Catalog # MAB4448) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Somatostatin R5/SSTR5

Somatostatin Receptor type 5 (SSTR5) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Within the extracellular portions, human SSTR5 shares 65% and 68% aa identity with mouse and rat SSTR5, respectively. It is expressed in adult pituitary, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. It is an inhibitory receptor that transduces the antiproliferative and antisecretive effects of somatostatins.

Long Name
Somatostatin Receptor Type 5
Entrez Gene IDs
6755 (Human); 20609 (Mouse); 25354 (Rat)
Alternate Names
Somatostatin R5; somatostatin receptor 5; somatostatin receptor subtype 5; somatostatin receptor type 5; SomatostatinR5; SS5R; SS-5-R; SS5-R; SST5; SSTR5

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