Somatostatin Receptor type 5 (SSTR5) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Within the extracellular portions, human SSTR5 shares 65% and 68% aa identity with mouse and rat SSTR5, respectively. It is expressed in adult pituitary, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. It is an inhibitory receptor that transduces the antiproliferative and antisecretive effects of somatostatins.
Human Somatostatin R5/SSTR5 Antibody
R&D Systems | Catalog # MAB4448
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 394401
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Somatostatin R5/SSTR5
Met1-Leu364
Accession # AAK61266
Met1-Leu364
Accession # AAK61266
Specificity
Detects human Somatostatin R5/SSTR5 in direct ELISAs. Stains Somatostatin R5/SSTR5 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Somatostatin R5/SSTR5 Antibody
Detection of Somatostatin R5/SSTR5 in HEK293 Human Cell Line Transfected with Human SSTR5 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Somatostatin R5/SSTR5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Somatostatin R5/SSTR5 Monoclonal Antibody (Catalog # MAB4448) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.Applications for Human Somatostatin R5/SSTR5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Somatostatin R5/SSTR5 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human Somatostatin R5/SSTR5 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Somatostatin R5/SSTR5
Long Name
Somatostatin Receptor Type 5
Alternate Names
SomatostatinR5, SST5, SSTR5
Gene Symbol
SSTR5
UniProt
Additional Somatostatin R5/SSTR5 Products
Product Documents for Human Somatostatin R5/SSTR5 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Somatostatin R5/SSTR5 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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