Intracellular Staining by Flow Cytometry
|Detection of SOX17 in Endoderm-differentiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells, endoderm differentiated with StemXVivo Endoderm Kit (Catalog # SC019), were stained with Mouse Anti-Human SOX17 PE‑conjugated Monoclonal Antibody (Catalog # IC19241P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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