Detection of SOX17 in Endoderm-differentiated BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells, endoderm differentiated with StemXVivo Endoderm Kit (Catalog # SC019), were stained with Mouse Anti-Human SOX17 PE‑conjugated Monoclonal Antibody (Catalog # IC19241P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
Kanai-Azuma, M. et al. (2002) Development 129:2367.
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