Human SOX17 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC19241P
Human SOX17 PE-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Product Details
Citations (2)
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Human SOX17 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 614013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of SOX17 in Endoderm-differentiated BG01V Human Cells by Flow Cytometry. View Larger

Detection of SOX17 in Endoderm-differentiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells, endoderm differentiated with StemXVivo Endoderm Kit (Catalog # SC019), were stained with Mouse Anti-Human SOX17 PE-conjugated Monoclonal Antibody (Catalog # IC19241P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

Product Datasheets

Citations for Human SOX17 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dynamic proteome profiling of human pluripotent stem cell-derived pancreatic progenitors
    Authors: LSW Loo, H Vethe, AAP Soetedjo, JA Paulo, J Jasmen, N Jackson, Y Bjørlykke, IA Valdez, M Vaudel, H Barsnes, SP Gygi, H Raeder, AKK Teo, RN Kulkarni
    Stem Cells, 2019;0(0):.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  2. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Authors: Q Liu, K Van Bortle, Y Zhang, MT Zhao, JZ Zhang, BS Geller, JJ Gruber, C Jiang, JC Wu, MP Snyder
    Sci Rep, 2018;8(1):12960.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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