Human STYK1 Alexa Fluor® 488-conjugated Antibody

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IC6668G-100UG
R&D Systems Antibodies
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Human STYK1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STYK1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 484713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human STYK1
Asp72-Val320
Accession # Q6J9G0
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STYK1

STYK1 (Ser/Thr/Tyr kinase 1; also NOK, or “novel oncogene with kinase-domain”) is a 50 kDa, distant member of the FGFR/PDGFR family of tyrosine kinases.  It appears to be intracellular, even though it contains a putative transmembrane segment.  Both MAPK and PI3K pathways are activated by STYK1 activity, and STYK1 is associated with oncogenesis.  Human STYK1 is 422 amino acids (aa) in length.  It contains 25 aa N-terminus, a 21 aa putative transmembrane segment, and a 396 aa C-terminus that contains a Tyr-kinase domain (aa 118-372).  Over aa 72-320, human STYK1 is 77% and 83% identical to mouse and canine STYK1, respectively.  

Long Name
Serine/Threonine/Tyrosine Kinase 1
Entrez Gene IDs
55359 (Human); 243659 (Mouse); 500340 (Rat)
Alternate Names
DKFZp761P1010; EC 2.7.10.2; NOK; NOKProtein PK-unique; serine/threonine/tyrosine kinase 1Novel oncogene with kinase domain; STYK1; SuRTK106; tyrosine-protein kinase STYK1

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