Human SUMO3 MAb (Clone 907614)

R&D Systems | Catalog # A-720

R&D Systems
Discontinued Product
A-720 has been discontinued. View all SUMO3 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 907614
View all SUMO3 Primary Antibodies »

Product Specifications

Immunogen

Peptide sequence from SUMO3
Accession # P55854

Specificity

This antibody detects endogenous, human SUMOylated proteins in Western blots. This antibody detects purified, recombinant SUMO3, but has no cross-reactivity to recombinant human SUMO1, 2, or 4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SUMO3 MAb (Clone 907614)

Detection of Human SUMO3 antibody by Western Blot.

Detection of Human SUMO3 by Western Blot.

Western blot shows lysates of SUMO3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO3 Monoclonal Antibody (Catalog # A-720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO3 at approximately 16-18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SUMO3 MAb (Clone 907614)

Application
Recommended Usage

Western Blot

0.1-0.5 µg/mL
Sample: Human SUMO3

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO3

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO3, also known as SMT3A, is synthesized as a 103 amino acid (aa), 11.5 kDa propeptide that contains an 11 aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO3 shares 83% sequence identity with SUMO3 from mouse. SUMO3 also has very high sequence homology to SUMO2 and SUMO4, 87% and 75%, respectively. SUMO3 shares only 47% sequence identity to SUMO1.

Long Name

Small Ubiquitin-like Modifier 3

Alternate Names

SMT3A, SMT3H1

Entrez Gene IDs

6612 (Human)

Gene Symbol

SUMO3

UniProt

P55854

Additional SUMO3 Products

Product Documents for Human SUMO3 MAb (Clone 907614)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SUMO3 MAb (Clone 907614)

For research use only

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