Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

R&D Systems | Catalog # IC5385F

R&D Systems
Discontinued Product
IC5385F has been discontinued. View all T-bet/TBX21 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Canine

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 525803
View all T-bet/TBX21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17

Specificity

Detects human T-bet.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

Detection of T-bet/TBX21 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC5385F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Entrez Gene IDs

30009 (Human); 57765 (Mouse); 303496 (Rat)

Gene Symbol

TBX21

UniProt

Q9UL17 (Human)

Additional T-bet/TBX21 Products

Product Documents for Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

For research use only

Citations for Human T‑bet/TBX21 Fluorescein‑conjugated Antibody

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