Human TAFA3/FAM19A3 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4677G-100UG
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Human TAFA3/FAM19A3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TAFA3/FAM19A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 4, or 5 is observed.
Source
Monoclonal Mouse IgG2b Clone # 460904
Immunogen
E. coli-derived recombinant human TAFA3/FAM19A3
Ala31-Arg133
Accession # Q7Z5A8
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TAFA3/FAM19A3

TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha, sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.

Long Name
Family with Sequence Similarity 19, Member A3
Entrez Gene IDs
284467 (Human); 329731 (Mouse)
Alternate Names
Chemokine-like protein TAFA-3; FAM19A3; family with sequence similarity 19 (chemokine (C-C motif)-like), member A3; MGC138473; TAFA3; TAFA-3

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