Intracellular Staining by Flow Cytometry
Detection of TAFA3/FAM19A3 in A172 Human Cell Line by Flow Cytometry. |
A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA3/FAM19A3 Monoclonal Antibody (Catalog # MAB4677, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha, sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.
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