Human TAFA4/FAM19A4 Alexa Fluor® 350-conjugated Antibody

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Product Details

Human TAFA4/FAM19A4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Detects human TAFA4/FAM19A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 3, or 5 is observed.
Monoclonal Mouse IgG1 Clone # 480103
E. coli-derived recombinant human TAFA4
Accession # Q96LR4
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 350


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TAFA4/FAM19A4

TAFA4 (also FAM19A4) is a secreted, 12 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 140 amino acid (aa) precursor that contains a 35 aa signal sequence and a 105 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern Cx7CCx13CxCx14Cx11Cx4Cx5Cx10C, where C represents a conserved cysteine residue and x represents any noncysteine amino acid (1). Human TAFA4 is 90% aa identical to mouse TAFA4 (1). Real-time PCR analysis indicates that TAFA4 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the thalamus (1). TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1, 2). TAFAs may also control axonal sprouting following brain injury (1).

  1. Tang, Y.T. et al. (2004) Genomics 83:727.
  2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.
Long Name
Family with Sequence Similarity 19, Member A4
Entrez Gene IDs
151647 (Human); 320701 (Mouse)
Alternate Names
Chemokine-like protein TAFA-4; FAM19A4; family with sequence similarity 19 (chemokine (C-C motif)-like), member A4; FLJ25161; TAFA4; TAFA-4

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