Intracellular Staining by Flow Cytometry
Detection of TAFA4/FAM19A4 in A172 Human Cell Line by Flow Cytometry. |
A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA4/FAM19A4 Monoclonal Antibody (Catalog # MAB5099, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG
TAFA4 (also FAM19A4) is a secreted, 12 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 140 amino acid (aa) precursor that contains a 35 aa signal sequence and a 105 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern Cx7CCx13CxCx14Cx11Cx4Cx5Cx10C, where C represents a conserved cysteine residue and x represents any noncysteine amino acid (1). Human TAFA4 is 90% aa identical to mouse TAFA4 (1). Real-time PCR analysis indicates that TAFA4 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the thalamus (1). TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1, 2). TAFAs may also control axonal sprouting following brain injury (1).
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