Human Testican 2/SPOCK2 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB2328AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 327403
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Product Specifications

Specificity

Detects human Testican 2/SPOCK2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Testican 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Testican 2/SPOCK2 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Testican 2/SPOCK2

Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). Nonetheless, overexpression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7).

References

  1. Nagase, T. et al. (1996) DNA Res. 3:321.
  2. Vannahme, C. et al. (1999) J. Neurochem. 73:12.
  3. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  4. Okazaki, Y. et al. (2002) Nature 420:563.
  5. Nakada, M. et al. (2001) Cancer Res. 61:8896.
  6. Nakada, M. et al. (2003) Cancer Res. 63:3364.
  7. Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.

Alternate Names

KIAA0275, SPOCK2

Entrez Gene IDs

9806 (Human)

Gene Symbol

SPOCK2

UniProt

Additional Testican 2/SPOCK2 Products

Product Documents for Human Testican 2/SPOCK2 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Testican 2/SPOCK2 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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