Human TFPI Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2974N Datasheet / COA / SDS

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FAB2974N-100UG

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Human TFPI Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TFPI or recombinant human TFPI-2 is observed.

Source

Monoclonal Mouse IgG2a Clone # 374720

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2).

Long Name

Tissue Factor Pathway Inhibitor

Entrez Gene IDs

7035 (Human); 21788 (Mouse)

Alternate Names

convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor