Human TM4SF4 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human TM4SF4 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Detects human TM4SF4 in ELISAs.
Monoclonal Mouse IgG1 Clone # 832441
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human TM4SF4
Accession # P48230
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TM4SF4 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7998G, filled histogram) or isotype control antibody (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TM4SF4

TM4SF4 (transmembrane 4 L6 family member 4), also known as IL-TMP (intestine and liver tetraspanin membrane protein) is a member of the L6 tetraspanin family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia and periportal hepatocytes, with increased expression in hepatocellular carcinomas. It is thought to mediate proliferation and adhesion. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and 25-40 kDa for various glycosylated forms.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. It shares 88% aa sequence identity with mouse and rat TM4SF4.

Long Name
Transmembrane 4 L Six Family Member 4
Entrez Gene IDs
7104 (Human); 229302 (Mouse); 116467 (Rat)
Alternate Names
FLJ31015; ILTMP; il-TMP; ILTMPintestinal and liver (il) tetraspan membrane protein; Intestine and liver tetraspan membrane protein; TM4SF4; transmembrane 4 L six family member 4; transmembrane 4 L6 family member 4; transmembrane 4 superfamily member 4

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human TM4SF4 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet &alpha Cell Heterogeneity
    Authors: EH Ghazvini Z, Z Huang, J Xia, D Li, HW Davidson, WH Li
    Cell Rep, 2020;32(2):107904.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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