Human TM4SF4 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7998G-100UG
Detection  of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry.
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Human TM4SF4 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TM4SF4 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 832441
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TM4SF4
Accession # P48230
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. View Larger

Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7998G, filled histogram) or isotype control antibody (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.        

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TM4SF4

TM4SF4 (transmembrane 4 L6 family member 4), also known as IL-TMP (intestine and liver tetraspanin membrane protein) is a member of the L6 tetraspanin family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia and periportal hepatocytes, with increased expression in hepatocellular carcinomas. It is thought to mediate proliferation and adhesion. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and 25-40 kDa for various glycosylated forms.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. It shares 88% aa sequence identity with mouse and rat TM4SF4.

Long Name
Transmembrane 4 L Six Family Member 4
Entrez Gene IDs
7104 (Human); 229302 (Mouse); 116467 (Rat)
Alternate Names
FLJ31015; ILTMP; il-TMP; ILTMPintestinal and liver (il) tetraspan membrane protein; Intestine and liver tetraspan membrane protein; TM4SF4; transmembrane 4 L six family member 4; transmembrane 4 L6 family member 4; transmembrane 4 superfamily member 4

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