Human TM4SF4 PE-conjugated Antibody

FAB7998P has been discontinued. View all TM4SF4 products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human TM4SF4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 832441
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TM4SF4
    Accession # P48230
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 PE‑conjugated Monoclonal Antibody (Catalog # FAB7998P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TM4SF4
TM4SF4 (Transmembrane 4 L6 Family Member 4), also known as IL-TMP (Intestine and Liver Tetraspanin Membrane Protein), is a distant member of the tetraspanin L6 domain family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia of the jejunum, periportal hepatocytes, and follicle-associated epithelium of the ileum. It is thought to mediate proliferation and adhesion, and is now known to complex with THTR-2 (Thiamine Transporter-2) during the transport of thiamine into expressing cells. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and varies from 25-30 kDa on proliferating cells, to 25-40 kDa on non-proliferating cells.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. The extracellular region of human TM4SF4 shares 72% aa sequence identity with mouse TM4SF4.
  • Long Name:
    Transmembrane 4 L Six Family Member 4
  • Entrez Gene IDs:
    7104 (Human); 229302 (Mouse); 116467 (Rat)
  • Alternate Names:
    FLJ31015; ILTMP; il-TMP; ILTMPintestinal and liver (il) tetraspan membrane protein; Intestine and liver tetraspan membrane protein; TM4SF4; transmembrane 4 L six family member 4; transmembrane 4 L6 family member 4; transmembrane 4 superfamily member 4
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