Human TMEM119 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB10313R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
Detection of TMEM119 in HEK293 Human Cell Line transfected with Human TMEM119 and eGFP by Flow Cytometry.
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Product Details
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Human TMEM119 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects human TREM-119 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 1023426
Protein A or G purified from cell culture supernatant
Chinese Hamster Ovary cell line, CHO derived human TREM-119
Accession # Q4V9L6.1
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line transfected with Human TMEM119 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of TMEM119 in HEK293 Human Cell Line transfected with Human TMEM119 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TMEM119 and eGFP was stained with (A) Mouse anti-human TMEM119 Alexa Fluor® 647-conjugated monoclonal antibody (Catalog # FAB10313R) or (B) Mouse IgG1 isotype control antibody (IC002R). Staining was performed using our Staining Membrane-Associated Proteins protocol.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TMEM119

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3). Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).

  1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathol. 36:39.
  4. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
Long Name
Transmembrane Protein 119
Entrez Gene IDs
338773 (Human); 231633 (Mouse)
Alternate Names
OBIF; Osteoblast Induction Factor; TMEM119

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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