Human TNF RI/TNFRSF1A PerCP-conjugated Antibody

Name: Human TNF RI/TNFRSF1A PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB225C
Price: 349 USD

Catalog #: FAB225C Datasheet / COA / SDS

Newer Version Available: FAB225G

NEW

Discontinued Product

FAB225C has been discontinued and is replaced by FAB225G.

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.

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All TNF RI/TNFRSF1A Antibodies

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Human TNF RI/TNFRSF1A PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII.

Source

Monoclonal Mouse IgG₁ Clone # 16803

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A PerCP-conjugated Monoclonal Antibody (Catalog # FAB225C, filled histogram) or isotype control antibody (Catalog # IC002C, open histo-gram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name

Tumor Necrosis Factor Receptor I

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Alternate Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor