Human TRAIL R2/TNFRSF10B Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6311R-025
Detection of TRAIL R2/TNFRSF10B in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Human TRAIL R2/TNFRSF10B Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.
Source
Monoclonal Mouse IgG2B Clone # 71908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Glu182
Accession # O14763
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TRAIL R2/TNFRSF10B in MDA‑MB‑231 Human Cell Line by Flow Cytometry. View Larger

Detection of TRAIL R2/TNFRSF10B in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB6311R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5, CD262, and TRICK 2, is a type 1 transmembrane TNFR family protein which serves as a receptor for TRAIL (APO2 Ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid (aa) residue precursor protein that contains extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, a molecule with which it shares 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4 (which tranduces apoptosis signaling) and two TRAIL decoy receptors (which antagonize TRAIL-induced apoptosis) have been reported. Human and Mouse TNFRSF10B extracellular domains share <40% aa sequence identity.

Long Name
TRAIL Receptor 2
Entrez Gene IDs
8795 (Human); 21933 (Mouse)
Alternate Names
CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9

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