Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6302A
Human TRAIL R3/TNFRSF10C APC-conjugated Antibody in Data
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Human TRAIL R3/TNFRSF10C APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRAIL R3/TNFRSF10C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R1, or rhTRAIL R4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 90906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
Met1-Ala221
Accession # O14798
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TRAIL R3/TNFRSF10C antibody in Human PBMC Monocytes antibody by Flow Cytometry. View Larger

Detection of TRAIL R3/TNFRSF10C in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human TRAIL R3/TNFRSF10C APC-conjugated Monoclonal Antibody (Catalog # FAB6302A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of TRAIL R3/TNFRSF10C antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of TRAIL R3/TNFRSF10C in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TRAIL R3/TNFRSF10C APC-conjugated Monoclonal Antibody (Catalog # FAB6302A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL R3/TNFRSF10C

Human TRAIL R3, also known as DcR1 (Decoy Receptor 1), LIT, and TRID, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. In the new TNF superfamily nomenclature, TRAIL R3 is referred to as TNFRSF10C. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References
  1. Sheridan, J.P. et al. (1997) Science 277:818.
  2. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 3
Entrez Gene IDs
8794 (Human)
Alternate Names
Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL R3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

Product Datasheets

Citation for Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells
    Authors: A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold
    PLoS ONE, 2019;14(4):e0214847.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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