Human TrkA APC-conjugated Antibody Summary
Ala33-Glu407
Accession # P04629
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TrkA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC-conjugated Monoclonal Antibody (Catalog # FAB1751A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry. BAF cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA APC-conjugated Monoclonal Antibody (Catalog # FAB1751A) or (B) Mouse IgG1 APC-conjugated isotype control antibody (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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TrkA Specificity is Shown by Flow Cytometry in Knockout Cell Line TrkA knockout K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC-conjugated Monoclonal Antibody (Catalog # FAB1751A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the TrkA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: TrkA
TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC. TrkA binds NGF and NT-3, while TrKB binds NT-4 and BDNF, and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of two cysteine-rich segments, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, coupled to an intracellular region that contains a tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid (aa) insertion after Pro392 in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues, whereas the shorter TrkA is expressed mainly in non-neuronal tissues. A third TrkA variant is expressed by neural stem cells. This isoform shows a Val substitution for aa 194-283 and an absence of the aforementioned 6 aa insert. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk. Over aa 33-407, human TrkA shares 79% aa sequence identity with mouse TrkA.
Product Datasheets
Citations for Human TrkA APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Nanoparticles increase human bronchial epithelial cell susceptibility to respiratory syncytial virus infection via nerve growth factor-induced autophagy
Authors: S Chakrabort, V Castranova, MK Perez, G Piedimonte
Physiol Rep, 2017-07-11;5(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Humoral activity of cord blood-derived stem/progenitor cells: implications for stem cell-based adjuvant therapy of neurodegenerative disorders.
Authors: Paczkowska E, Kaczynska K, Pius-Sadowska E, Roginska D, Kawa M, Ustianowski P, Safranow K, Celewicz Z, Machalinski B
PLoS ONE, 2013-12-31;8(12):e83833.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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