Human TrkA APC-conjugated Antibody

  (2 citations)     
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Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human TrkA in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant rat TrkA, recombinant human (rh) TrkB, or rhTrkC.
  • Source
    Monoclonal Mouse IgG1 Clone # 165131
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TrkA
    Ala33-Glu407
    Accession # P04629
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of TrkA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC‑conjugated Monoclonal Antibody (Catalog # FAB1751A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC. TrkA binds NGF and NT-3, while TrKB binds NT-4 and BDNF, and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of two cysteine-rich segments, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, coupled to an intracellular region that contains a tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid (aa) insertion after Pro392 in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues, whereas the shorter TrkA is expressed mainly in non-neuronal tissues. A third TrkA variant is expressed by neural stem cells. This isoform shows a Val substitution for aa 194-283 and an absence of the aforementioned 6 aa insert. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk. Over aa 33-407, human TrkA shares 79% aa sequence identity with mouse TrkA.

  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor A
  • Entrez Gene IDs:
    4914 (Human); 59109 (Rat)
  • Alternate Names:
    DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Nanoparticles increase human bronchial epithelial cell susceptibility to respiratory syncytial virus infection via nerve growth factor-induced autophagy
    Authors: S Chakrabort, V Castranova, MK Perez, G Piedimonte
    Physiol Rep, 2017;5(13):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Humoral activity of cord blood-derived stem/progenitor cells: implications for stem cell-based adjuvant therapy of neurodegenerative disorders.
    Authors: Paczkowska E, Kaczynska K, Pius-Sadowska E, Roginska D, Kawa M, Ustianowski P, Safranow K, Celewicz Z, Machalinski B
    PLoS ONE, 2013;8(12):e83833.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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Isotype Controls
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Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 11  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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