Detection of TrkA in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC‑conjugated Monoclonal Antibody (Catalog # FAB1751A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC. TrkA binds NGF and NT-3, while TrKB binds NT-4 and BDNF, and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of two cysteine-rich segments, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, coupled to an intracellular region that contains a tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid (aa) insertion after Pro392 in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues, whereas the shorter TrkA is expressed mainly in non-neuronal tissues. A third TrkA variant is expressed by neural stem cells. This isoform shows a Val substitution for aa 194-283 and an absence of the aforementioned 6 aa insert. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk. Over aa 33-407, human TrkA shares 79% aa sequence identity with mouse TrkA.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.