Detects human Tryptase gamma ‑1/TPSG1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCPT-1, recombinant human (rh) Tryptase alpha, rmTryptase beta -1, rhTryptase beta -2, or rmTryptase-5 is observed.
Monoclonal Rat IgG2B Clone # 279005
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Tryptase gamma ‑1/TPSG1 Arg20-Arg281 Accession # Q9NRR2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase gamma-1/TPSG1
As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including tryptases, chymase and cathepsin G (4). Tryptase gamma -1, also called transmembrane tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma ‑1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2).
Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.
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