Rat IgG2B Isotype Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Detection of Rat IgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rat IgG2B Isotype Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Interferon-?3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection
Authors: SH Ferguson, DM Foster, B Sherry, ST Magness, DM Nielsen, JL Gookin
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Porcine
Sample Types: In Vivo
Applications: Neutralization -
Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice
Authors: M Takehara, Y Sonobe, H Bandou, K Kobayashi, M Nagahama
Toxins (Basel), 2019;11(9):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Integrin-based diffusion barrier separates membrane domains enabling formation of microbiostatic frustrated phagosomes
Authors: ME Maxson, X Naj, TR O'Meara, JD Plumb, LE Cowen, S Grinstein
Elife, 2018;7(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Imiquimod-Induced Psoriasis-Like Skin Lesions Do Not Accelerate Atherosclerosis in Low Density Lipoprotein Receptor-Deficient Mice
Authors: M Madsen, PR Hansen, LB Nielsen, RM Cardoso, M van Eck, TX Pedersen
Am. J. Pathol., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
J Innate Immun, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
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The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea
Authors: MJ Lopez, Y Seyed-Raza, A Jamali, DL Harris, P Hamrah
Invest. Ophthalmol. Vis. Sci., 2018;59(13):5671-5681.
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype Control -
NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
J. Cell. Mol. Med, 2017;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima
Nat Immunol, 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
Physiol Rep, 2016;4(23):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis.
Authors: Kowalewska P, Nguyen U, Burrows L, Fox-Robichaud A
J Biomed Sci, 2016;23(1):20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Visualized macrophage dynamics and significance of S100A8 in obese fat.
Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
Proc Natl Acad Sci U S A, 2015;112(16):E2058-66.
Species: Mouse
Sample Types: Whole Tissue
Applications: in vitro -
Structure-function analysis of CCL28 in the development of post-viral asthma.
Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B
J Biol Chem, 2015;290(7):4528-36.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
Blood, 2014;123(12):1887-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Authors: Kowalewska P, Patrick A, Fox-Robichaud A
PLoS ONE, 2014;9(9):e104537.
Species: Mouse
Sample Types: In Vivo
Applications: IHC-Fr -
Oxidative stress induced age dependent meibomian gland dysfunction in Cu, Zn-superoxide dismutase-1 (Sod1) knockout mice.
Authors: Ibrahim O, Dogru M, Matsumoto Y, Igarashi A, Kojima T, Wakamatsu T, Inaba T, Shimizu T, Shimazaki J, Tsubota K
PLoS ONE, 2014;9(7):e99328.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Authors: Moore RN, Cherry JF, Mathur V
Stem Cells Dev., 2012;21(1):30-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
PLoS ONE, 2012;7(4):e34608.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
Authors: Oh MH, Oh SY, Yu J
J. Immunol., 2011;186(12):7232-42.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
Authors: Mendez MP, Monroy YK, Du M
Respir. Res., 2011;12(1):12.
Species: Mouse
Sample Types: BALF
Applications: Control -
Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
J. Biol. Chem., 2011;286(20):17435-44.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2011;186(9):5435-42.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.
Authors: Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S
J. Cell Biol., 2010;191(6):1205-18.
Species: Mouse
Sample Types:
Applications: Flow Cytometry Control -
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
J. Immunol., 2010;184(8):4159-69.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Authors: Narasaraju T, Ng HH, Phoon MC
Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
Authors: Mosconi E, Rekima A, Seitz-Polski B
Mucosal Immunol, 2010;3(5):461-74.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M
Blood, 2010;115(24):5102-10.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
Nat. Med., 2009;15(9):1016-22.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
J. Mol. Cell. Cardiol., 2006;41(5):885-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: Control -
CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model.
Authors: Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS
Int. Immunol., 2006;18(8):1233-42.
Species: Mouse
Sample Types:
Applications: Control -
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Control -
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infect. Immun., 2004;72(12):7247-56.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
J. Immunol., 2001;166(2):1233-40.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
Stem Cells Transl Med, 0;4(1):44-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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