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Rat IgG2B Isotype Control

MAB0061 42 Citations 6 Reviews Datasheet / COA / SDS
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MAB0061

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Detection of Rat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (42)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG2B Isotype Control Summary

Specificity
Selected for use as a rat IgG2B isotype control. In direct ELISAs, does not cross-react with 102 different cytokines.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Control Detection of Rat IgG Isotype Control by Flow Cytometry View Larger

Detection of Rat IgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Rat IgG2B Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
    Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
    Cell Reports, 2022;39(2):110670.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
    Applications: Neutralization Control
  2. Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
    Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
    Journal of Immunology Research, 2022;2022(0):9926305.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  3. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021;15(0):683687.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
    Authors: K Cosker, A Mallach, J Limaye, TM Piers, J Staddon, SJ Neame, J Hardy, JM Pocock
    Scientific Reports, 2021;11(1):13316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Tumor-infiltrating CD62L+PD-1-CD8 T cells retain proliferative potential via Bcl6 expression and replenish effector T cells within the tumor
    Authors: Y Gong, T Suzuki, H Kozono, M Kubo, N Nakano
    PLoS ONE, 2020;15(8):e0237646.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  6. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin
    Authors: D Andreev, M Liu, D Weidner, K Kachler, M Faas, A Grüneboom, U Schlötzer-, LE Muñoz, U Steffen, B Grötsch, B Killy, G Krönke, AM Luebke, A Niemeier, F Wehrhan, R Lang, G Schett, A Bozec
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces
    Authors: L Andreasson, H Evenbratt, R Mobini, S Simonsson
    J Biotechnol, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis
    Authors: RJ Napier, EJ Lee, MP Davey, EE Vance, JM Furtado, PE Snow, KA Samson, SJ Lashley, BR Brown, R Horai, MJ Mattapalli, B Xu, MC Callegan, LS Uebelhoer, CL Lancioni, RK Vehe, BA Binstadt, JR Smith, RR Caspi, HL Rosenzweig
    Nat Commun, 2020;11(1):5406.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Functional Assay
  9. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin
    Authors: D Andreev, M Liu, D Weidner, K Kachler, M Faas, A Grüneboom, U Schlötzer-, LE Muñoz, U Steffen, B Grötsch, B Killy, G Krönke, AM Luebke, A Niemeier, F Wehrhan, R Lang, G Schett, A Bozec
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice
    Authors: M Takehara, Y Sonobe, H Bandou, K Kobayashi, M Nagahama
    Toxins (Basel), 2019;11(9):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Interferon-?3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection
    Authors: SH Ferguson, DM Foster, B Sherry, ST Magness, DM Nielsen, JL Gookin
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Porcine
    Sample Types: In Vivo
    Applications: Neutralization
  12. The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea
    Authors: MJ Lopez, Y Seyed-Raza, A Jamali, DL Harris, P Hamrah
    Invest. Ophthalmol. Vis. Sci., 2018;59(13):5671-5681.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  13. Integrin-based diffusion barrier separates membrane domains enabling formation of microbiostatic frustrated phagosomes
    Authors: ME Maxson, X Naj, TR O'Meara, JD Plumb, LE Cowen, S Grinstein
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  14. Imiquimod-Induced Psoriasis-Like Skin Lesions Do Not Accelerate Atherosclerosis in Low Density Lipoprotein Receptor-Deficient Mice
    Authors: M Madsen, PR Hansen, LB Nielsen, RM Cardoso, M van Eck, TX Pedersen
    Am. J. Pathol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
    Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
    J Innate Immun, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  16. NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
    Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016;4(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis.
    Authors: Kowalewska P, Nguyen U, Burrows L, Fox-Robichaud A
    J Biomed Sci, 2016;23(1):20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Visualized macrophage dynamics and significance of S100A8 in obese fat.
    Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
    Proc Natl Acad Sci U S A, 2015;112(16):E2058-66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: in vitro
  21. Structure-function analysis of CCL28 in the development of post-viral asthma.
    Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B
    J Biol Chem, 2015;290(7):4528-36.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Oxidative stress induced age dependent meibomian gland dysfunction in Cu, Zn-superoxide dismutase-1 (Sod1) knockout mice.
    Authors: Ibrahim O, Dogru M, Matsumoto Y, Igarashi A, Kojima T, Wakamatsu T, Inaba T, Shimizu T, Shimazaki J, Tsubota K
    PLoS ONE, 2014;9(7):e99328.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Types: In Vivo
    Applications: IHC-Fr
  24. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
    Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
    Blood, 2014;123(12):1887-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2012;21(1):30-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  26. The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
    Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
    PLoS ONE, 2012;7(4):e34608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  27. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
    Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
    J. Biol. Chem., 2011;286(20):17435-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  28. IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
    Authors: Oh MH, Oh SY, Yu J
    J. Immunol., 2011;186(12):7232-42.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  29. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
    Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2011;186(9):5435-42.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  30. Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
    Authors: Mendez MP, Monroy YK, Du M
    Respir. Res., 2011;12(1):12.
    Species: Mouse
    Sample Types: BALF
    Applications: Control
  31. Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
    Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M
    Blood, 2010;115(24):5102-10.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  32. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  33. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010;184(8):4159-69.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  34. Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.
    Authors: Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S
    J. Cell Biol., 2010;191(6):1205-18.
    Species: Mouse
    Sample Types:
    Applications: Flow Cytometry Control
  35. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010;3(5):461-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  36. A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
    Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
    Nat. Med., 2009;15(9):1016-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  37. CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model.
    Authors: Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS
    Int. Immunol., 2006;18(8):1233-42.
    Species: Mouse
    Sample Types:
    Applications: Control
  38. Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
    Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Control
  39. A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
    Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
    J. Mol. Cell. Cardiol., 2006;41(5):885-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Control
  40. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004;72(12):7247-56.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  41. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
    Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
    J. Immunol., 2001;166(2):1233-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  42. Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
    Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
    Stem Cells Transl Med, 0;4(1):44-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the light chain of Rat IgG2B Isotype Control, Catalog # MAB0061?

    • The light chain is kappa. 

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Reviews for Rat IgG2B Isotype Control

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Rat IgG2B Isotype Control
By Abby Sukarto on 02/13/2020
Application: Flow Cytometry Sample Tested: Bone Extracts Species: Human
Flow Cytometry Rat IgG2B Isotype Control MAB0061
Rat IgG2B Isotype Control
By Anonymous on 09/28/2019
Application: Gel Shift Sample Tested: Purified protein Species: Rat
Gel Shift Rat IgG2B Isotype Control MAB0061
Rat IgG2B Isotype Control
By Abby Sukarto on 09/10/2019
Sample Tested: Bone Extracts,bone marrow Species: Human

Need optimization of your specific cell types.

Rat IgG2B Isotype Control
By Ju Shi on 12/01/2017
Application: Flow Cytometry Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Rat IgG2B Isotype Control
By Anonymous on 09/01/2016
Application: Flow Cytometry Sample Tested: Bone marrow cells Species: Mouse
Rat IgG2B Isotype Control
By Anonymous on 07/11/2016
Application: Functional Assay Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse

72 hours cytotoxicity assay using secondary Zap conjugate

Functional Assay Rat IgG2B Isotype Control MAB0061