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Rat IgG2B Isotype Control

  (28 citations)
(3 Reviews)     
Datasheet / CoA / SDS
Detection of Rat IgGControl by Flow Cytometry

Control

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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews
  • Specificity
    Selected for use as a rat IgG2B isotype control. In direct ELISAs, does not cross-react with 102 different cytokines.
  • Source
    Monoclonal Rat IgG2B Clone # 141945
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Control
Control Rat IgG2b Isotype Control (141945) [Unconjugated]
click image to view larger
Detection of Rat IgGControl by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Rat Anti-Mouse CD44 Monoclonal Antibody (Catalog # MAB6127,filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB0061,open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Species
 Applications
 Sample Type
  1. Integrin-based diffusion barrier separates membrane domains enabling formation of microbiostatic frustrated phagosomes
    Authors: ME Maxson, X Naj, TR O'Meara, JD Plumb, LE Cowen, S Grinstein
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  2. NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
    Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016;4(23):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  5. Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis.
    Authors: Kowalewska P, Nguyen U, Burrows L, Fox-Robichaud A
    J Biomed Sci, 2016;23(1):20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Structure-function analysis of CCL28 in the development of post-viral asthma.
    Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B
    J Biol Chem, 2015;290(7):4528-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Visualized macrophage dynamics and significance of S100A8 in obese fat.
    Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
    Proc Natl Acad Sci U S A, 2015;112(16):E2058-66.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: in vitro
  8. Oxidative stress induced age dependent meibomian gland dysfunction in Cu, Zn-superoxide dismutase-1 (Sod1) knockout mice.
    Authors: Ibrahim O, Dogru M, Matsumoto Y, Igarashi A, Kojima T, Wakamatsu T, Inaba T, Shimizu T, Shimazaki J, Tsubota K
    PLoS ONE, 2014;9(7):e99328.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  9. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Type: In Vivo
    Application: IHC Fresh
  10. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
    Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
    Blood, 2014;123(12):1887-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2012;21(1):30-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  12. The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
    Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
    PLoS ONE, 2012;7(4):e34608.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  13. IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
    Authors: Oh MH, Oh SY, Yu J
    J. Immunol., 2011;186(12):7232-42.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  14. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
    Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2011;186(9):5435-42.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  15. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
    Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
    J. Biol. Chem., 2011;286(20):17435-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  16. Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
    Authors: Mendez MP, Monroy YK, Du M
    Respir. Res., 2011;12(1):12.
    Species: Mouse
    Sample Type: BALF
    Application: Ctrl
  17. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010;3(5):461-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  18. Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.
    Authors: Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S
    J. Cell Biol., 2010;191(6):1205-18.
    Species: Mouse
    Sample Type:
    Application: Flow control
  19. Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
    Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M
    Blood, 2010;115(24):5102-10.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  20. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010;184(8):4159-69.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  21. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
    Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
    Nat. Med., 2009;15(9):1016-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  23. A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
    Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
    J. Mol. Cell. Cardiol., 2006;41(5):885-92.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Ctrl
  24. Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
    Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Ctrl
  25. CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model.
    Authors: Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS
    Int. Immunol., 2006;18(8):1233-42.
    Species: Mouse
    Sample Type:
    Application: Ctrl
  26. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004;72(12):7247-56.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  27. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
    Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
    J. Immunol., 2001;166(2):1233-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  28. Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
    Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
    Stem Cells Transl Med, 0;4(1):44-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay - Ctrl
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Average Rating: 4 (Based on 3 reviews)

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We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 applications: Flow Cytometry, Functional Assay.

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Images Ratings Applications Species Reviewed By Date Details
Excellent
  Flow Human Anonymous 12/01/2017
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Summary

Application Flow Cytometry
Sample Tested HEK293 human embryonic kidney cell line
Species Human
Very Good
  Flow Mouse Anonymous 09/01/2016
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Summary

Application Flow Cytometry
Sample Tested Bone marrow cells
Species Mouse
Functional Assay Rat IgG2B Isotype Control MAB0061 Array MAB0061
Enlarge 		Average
  Functional Assay Mouse Anonymous 07/11/2016
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Image Details
Functional Assay Rat IgG2B Isotype Control MAB0061
Functional Assay: Rat IgG2B Isotype Control [MAB0061].

Summary

Application Functional Assay
Sample Tested RAW 264.7 mouse monocyte/macrophage cell line
Species Mouse

Other Experimental Details

Other Experimental Details 72 hours cytotoxicity assay using secondary Zap conjugate

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