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Human UbcH2/UBE2H Polyclonal Ab

Catalog #: A-605
1 Citation Datasheet / COA / SDS
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A-605 has been discontinued.
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Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UbcH2/UBE2H Polyclonal Ab Summary

Species Reactivity
Human
Specificity
Detects UbcH2/UBE2H in Western blots.
Source
Polyclonal Rabbit IgG
Immunogen
Full-length, highly purified human UbcH2 protein
Formulation
Supplied in a solution of PBS and Glycerol.

Applications

Recommended Concentration
Sample
Western Blot
Recommendeddilution range for Western blot analysis is 1:100 to 1:1000.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UbcH2/UBE2H

The UbcH2 enzyme encodes for the human homolog of the yeast Ubc8.  UbcH2 can conjugate ubiquitin to histone H2A in an E3-independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates.  Additionally, UbcH2 may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Entrez Gene IDs
7328 (Human); 22214 (Mouse)
Alternate Names
E2-14K; E2-20K; EC 6.3.2.19; UBC8; UBC8E2-20K; UBCH; UbcH2; UBE2H; Ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2 H; Ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); Ubiquitin-protein ligase H

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Citation for Human UbcH2/UBE2H Polyclonal Ab

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma
    Authors: MC Yen, KL Wu, YW Liu, YY Chang, CY Chang, JY Hung, YM Tsai, YL Hsu
    Biology, 2021-04-28;10(5):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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