Human VSIG2 Antibody

R&D Systems | Catalog # MAB5177

R&D Systems
Discontinued Product
MAB5177 has been discontinued. View all VSIG2 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 541527
View all VSIG2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VSIG2
Val24-Ala243
Accession # Q96IQ7

Specificity

Detects human VSIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VSIG1, 3, 4, or recombinant mouse VSIG2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VSIG2 Antibody

Detection of Human VSIG2 antibody by Western Blot.

Detection of Human VSIG2 by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG2 Monoclonal Antibody (Catalog # MAB5177) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VSIG2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human VSIG2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human prostate tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG2

VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Human VSIG2 precursor is 327  amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 24‑243) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 24‑137) and one C2-type Ig-like domain (aa 144‑233). One potential splice variant exists that shows a deletion of aa 285‑327. Over aa 24‑243, human VSIG2 is 85% aa identical to mouse VSIG2.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Alternate Names

CTH, CTXL

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Gene Symbol

VSIG2

UniProt

Q96IQ7

Additional VSIG2 Products

Product Documents for Human VSIG2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VSIG2 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

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