Human VSIG2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human VSIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VSIG1, 3, 4, or recombinant mouse VSIG2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 541527
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human VSIG2
    Val24-Ala243
    Accession # Q96IQ7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human VSIG2 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG2 Monoclonal Antibody (Catalog # MAB5177) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VSIG2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG2
VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Human VSIG2 precursor is 327  amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 24‑243) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 24‑137) and one C2-type Ig-like domain (aa 144‑233). One potential splice variant exists that shows a deletion of aa 285‑327. Over aa 24‑243, human VSIG2 is 85% aa identical to mouse VSIG2.
  • Long Name:
    V-Set and Immunoglobulin Domain Containing 2
  • Entrez Gene IDs:
    23584 (Human); 57276 (Mouse); 300520 (Rat)
  • Alternate Names:
    2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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