Human VSIG3 Antibody

Detection of Human VSIG3 by Simple Western^TM.

Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for VSIG3 at approximately 57 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of VSIG3 by Flow Cytometry

Human and mouse MB tumor cells exhibit in vitro expression of VISTA binding partners and are translationally relevant. (a) Cells gated on live singlets, then further gated based on GFP expression. Histograms (blue) representing in vitro expression of VSIG8 and VSIG3 in human MB cell lines (D283-MED and D425-MED) and our GFP+ mouse medulloblastoma cell line (mCB DNp53 MYC). Fluorescence minus one control represented in gray, and isotype control represented in green. (b) Western blot analysis confirming expression of VSIG3 and VSIG8 and relative absence of VISTA from human and mouse medulloblastoma tumor cell lines (n = 3). Uncropped blots are shown in File S2. (c) Transcriptome analysis of 52 human MB patient samples. X-axis represents quartile distribution of VISTA expression levels: Q1 (lowest 25%), Q2 (next 25%), Q3 (third 25%), and Q4 (highest 25%). Y-axis represents log2 [TPM+1]. Bar graphs representing VISTA transcript (C10orf54) expression correlation with VSIG3, VSIG8, and PSGL1 and LRIG1. Error bars represent standard error of the mean (SEM), and p-values were calculated using a Mann–Whitney test and corrected for multiple comparisons. The asterisks indicate adjusted p-values as follows: * padj < 0.05, ** padj < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39123357), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human VSIG3 by Western Blot.

Western blot shows lysates of IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.