Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Llama VHH domain Clone # Hu142
View all vWF-A1 Primary Antibodies »

Product Specifications

Immunogen

vWF-A1 protein

Specificity

VHH antibody detecting vWF-A1

Clonality

Monoclonal

Host

Llama

Isotype

VHH domain

Scientific Data Images for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Detection of vWF-A1 in HUVECS cells by Flow Cytometry.

HUVECS cells treated with 5 ug/mL Brefeldin A for 5 hours were stained with Human vWF-A1 (RUO grade Caplacizumab Biosimilar) Antibody (Catalog # MAB10591, Filled histogram) or isotype control antibody Llama IgG (open histogram), followed by Goat anti Alpaca Rhodamine Red. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC Human umbilical vein endothelial cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: vWF-A1

Caplacizumab is a humanized VHH immunoglobulin that binds to the A1 domain of the large von Willdebrand factor resulting in the inhibition of the interaction of glycoprotein GPIb-IX-V receptor on platelet surface.  The result of neutralizing of the von Willedebrand factor is reduced platelet aggregation.  Caplacizumab has been approved for the treatment of acute thrombotic thrombocytopenic purpura (aTTP), and has been shown to reduce the occurrence of thromboembolic events.

Long Name

von Willebrand Factor A1 Domain

Alternate Names

vWFA1

Entrez Gene IDs

7450 (Human)

Gene Symbol

VWF

Additional vWF-A1 Products

Product Documents for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

For research use only

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