Human XBP1 (S Isoform) Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4257G-100UG
Product Details
FAQs
Reviews

Human XBP1 (S Isoform) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).
Source
Monoclonal Mouse IgG1 Clone # 525904
Immunogen
E. coli-derived recombinant human XBP1S
Ser188-Leu287
Accession # NP_001073007
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
RPMI 8226 human multiple myeloma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: XBP1

X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.

Long Name
X-box Binding Protein 1
Entrez Gene IDs
7494 (Human); 22433 (Mouse); 289754 (Rat)
Alternate Names
HTF; TREB5; X-box binding protein 1; X-box-binding protein 1; XBP1; XBP-1; XBP2; XBP2Tax-responsive element-binding protein 5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human XBP1 (S Isoform) Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human XBP1 (S Isoform) Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Human XBP1 (S Isoform) Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review