Human XBP1 (S Isoform) Antibody Summary
Accession # NP_001073007
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.
Citations for Human XBP1 (S Isoform) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Induction of autophagy-dependent apoptosis in cancer cells through activation of ER stress: an uncovered anti-cancer mechanism by anti-alcoholism drug disulfiram
Authors: X Zhang, P Hu, SY Ding, T Sun, L Liu, S Han, AB DeLeo, A Sadagopan, W Guo, X Wang
Am J Cancer Res, 2019;9(6):1266-1281.
Sample Types: Whole Cells
Applications: Flow Cytometry
In vivo crystallization of human IgG in the endoplasmic reticulum of engineered Chinese hamster ovary (CHO) cells.
Authors: Hasegawa H, Wendling J, He F
J. Biol. Chem., 2011;286(22):19917-31.
Sample Types: Whole Cells
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