Human XBP1 (S Isoform) Antibody (525904)

R&D Systems | Catalog # MAB4257

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 525904
View all XBP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XBP1S
Ser188-Leu287
Accession # NP_001073007

Specificity

Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human XBP1 (S Isoform) Antibody (525904)

Detection of XBP1S antibody in RPMI 8226 Human Cell Line antibody by Flow Cytometry.

Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry.

RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human XBP1 (S Isoform) Antibody (525904)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: RPMI 8226 human multiple myeloma cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 5 using MAB4257 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XBP1

X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.

Long Name

X-box Binding Protein 1

Alternate Names

HTF, TREB5, XBP2

Entrez Gene IDs

7494 (Human); 22433 (Mouse); 289754 (Rat)

Gene Symbol

XBP1

UniProt

NP_001073007

Additional XBP1 Products

Product Documents for Human XBP1 (S Isoform) Antibody (525904)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human XBP1 (S Isoform) Antibody (525904)

For research use only

Citations for Human XBP1 (S Isoform) Antibody (525904)

Customer Reviews for Human XBP1 (S Isoform) Antibody (525904) (1)

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Customer Images

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  • Name: Natalie Erdmann
    Application: Immunofluorescence
    Sample Tested: Multiple myeloma cells lines MY5 & JJN3 with and without XBP1s-GFP transfection
    Species: Human
    Verified Customer | Posted 08/17/2015
    IFF/ICC staining of JJN3 and MY5 cells with MAB4257
    Human XBP1 (S Isoform) Antibody (525904) MAB4257

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