Human XCR1 Alexa Fluor® 700-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human XCR1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human XCR1
    Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
    Accession # P46094
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of XCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Goat Anti-Human XCR1 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB857N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of XCR1 in NS0 Mouse Cell Line Transfected with Human XCR1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human XCR1 and eGFP was stained with either (A) Goat Anti-Human XCR1 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB857N) or (B) Normal Goat IgG Alexa Fluor 700 Control (Catalog # IC108N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

  • Long Name:
    Chemokine [C Motif] Receptor 1
  • Entrez Gene IDs:
    2829 (Human); 23832 (Mouse); 301086 (Rat)
  • Alternate Names:
    CCXCR1; CCXCR1XC chemokine receptor 1; chemokine (C motif) receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; GPR5chemokine (C motif) XC receptor 1; G-protein coupled receptor 5; Lymphotactin receptor; XCR1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Alexa Fluor® 700-conjugated Control

Ctrl IC108N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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