Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

Novus Biologicals | Catalog # NBP2-03361

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C3
View all Hydroxyacid Oxidase-1/HAO-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HAO1 (NP_060015) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

Western Blot: HAO1 Antibody (5C3) [NBP2-03361] Analysis of extracts (35ug) from 9 different cell lines by using anti-HAO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3) [NBP2-03361]

Flow Cytometry: Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3) [NBP2-03361]

Flow Cytometry: HAO1 Antibody (5C3) [NBP2-03361] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HAO1 antibody, and then analyzed by flow cytometry.
Western Blot: Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3) [NBP2-03361]

Western Blot: Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3) [NBP2-03361]

Western Blot: HAO1 Antibody (5C3) [NBP2-03361] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1.

Applications for Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:1000-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hydroxyacid Oxidase-1/HAO-1

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]. COMPLETENESS: full length.

Alternate Names

GOX, HAO-1, HAO1, HAOX1, Hydroxyacid Oxidase1

Entrez Gene IDs

54363 (Human)

Gene Symbol

HAO1

Additional Hydroxyacid Oxidase-1/HAO-1 Products

Product Documents for Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

Certificate of Analysis

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Product Specific Notices for Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

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