IBG3

Tocris Bioscience | Catalog # 8129

Potent molecular glue Degrader of BET proteins BRD4 and BRD2
Tocris Bioscience

Key Product Details

Description

Potent molecular glue Degrader of BET proteins BRD4 and BRD2
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Product Description

IBG3 is a very potent JQ1-containing molecular glue Degrader of BET proteins BRD4 and BRD2 (DC50 values are 6.7 pM and 8.6 pM respectively). Induces proximity between BRD4 and DCAF16 to form a ternary complex (EC50 = 32 nM).

Scientific Data Images for IBG3

Published vs Bio-Techne batch comparison for IBG3

Published vs Bio-Techne batch comparison for IBG3.

Endogenous HiBiT-BRD4 HEK293 cells were plated at a density of 20,000 cell per well in 100 µL of DMEM (10% FBS) in a white-walled 96 well plate and left overnight to adhere at 37 ºC, 5% CO2. Cells were incubated in 50 µL of 10 nM-0.1 pM of IBG3 or vehicle control (DMSO)  in Optimem (10% FBS) and left for 4 hours. Cells were then treated with HiBIT Lytic assay reagent– and plates were then read on a BMG Pherastar plate reader for luminescence detection. Data kindly provided by the Ciulli laboratory, University of Dundee.

Product Specifications for IBG3

Molecular Weight

976.23

Formula

C54H57N9O5S2

Storage

Store at -20°C

Chemical Name

tert-Butyl 2-((S)-4-(4-((4'-((S)-6-(2-(tert-butoxy)-2-oxoethyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-4-yl)-[1,1'-biphenyl]-4-carboxamido)methyl)phenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate

PubChem ID

171037958

InChI Key

MRIUQCAXSFYTQZ-YATWDLPUSA-N

SMILES

CC1=C(SC2=C1C(C3=CC=C(C=C3)C4=CC=C(C=C4)C(NCC5=CC=C(C6=N[C@H](C7=NN=C(N7C8=C6C(C)=C(S8)C)C)CC(OC(C)(C)C)=O)C=C5)=O)=N[C@H](C9=NN=C(N92)C)CC(OC(C)(C)C)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 97.62 100

Preparing Stock Solutions for IBG3

The following data is based on the product molecular weight 976.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.02 mL 5.12 mL 10.24 mL
5 mM 0.20 mL 1.02 mL 2.05 mL
10 mM 0.10 mL 0.51 mL 1.02 mL
50 mM 0.02 mL 0.10 mL 0.20 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for IBG3

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IBG3

For research use only

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