Catalog Number: 1531
Alternate Names: AG 3-5
Chemical Name: 3,4-Dihydro-3-(2-hydroxyphenyl)-6-(3-nitrophenyl)-(1H)-pyrimidin-2-one
Biological Activity
Cooling agent that activates the novel cold receptors TRPM8 (CMR1) and TRPA1 (ANKTM1/TRPN1), members of the TRP ion channel family. Induces currents in CMR1-expressing HEK 293 cells (EC50 = 0.36 μM) more potently than menthol or low temperatures. Produces "wet shakes" in vivo.
Technical Data
  • M.Wt:
    311.3
  • Formula:
    C16H13N3O4
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at RT
  • CAS No:
    36945-98-9
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay.
    Behrendt et al.
    Br.J.Pharmacol., 2004;141:737
  2. Identification of a cold receptor reveals a general role for TRP channels in thermosensation.
    McKemy et al.
    Nature, 2002;416:52
  3. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures.
    Story et al.
    Cell, 2003;112:819
  4. AG-3-5: a chemical producing sensations of cold.
    Wei et al.
    J.Pharm.Pharmacol., 1983;35:110
Citations:

The citations listed below are publications that use Tocris products. Selected citations for Icilin include:

3 Citations: Showing 1 - 3
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  1. Inhibitory Interneurons That Express GFP in the PrP-GFP Mouse Spinal Cord Are Morphologically Heterogeneous, Innervated by Several Classes of Primary Afferent and Include Lamina I Projection Neurons among Their Postsynaptic Targets.
    Authors: Ganley Et al.
    Oncogene 2015;35:7626
  2. PSA reduces prostate cancer cell motility by stimulating TRPM8 activity and plasma membrane expression.
    Authors: Gkika Et al.
    Respir Res 2010;29:4611
  3. Analgesia mediated by the TRPM8 cold receptor in chronic neuropathic pain.
    Authors: Proudfoot Et al.
    Curr Biol 2006;16:1591

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