IDH2 Antibody (8C3D4)

Novus Biologicals | Catalog # NBP3-15366

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8C3D4 expressed in HEK293
View all IDH2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 353-452 of human IDH2 (P48735). RHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IDH2 Antibody (8C3D4)

Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366]

Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366]

Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of NIH-3T3 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366]

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of C6 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for IDH2 Antibody (8C3D4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Gene Symbol

IDH2

Additional IDH2 Products

Product Documents for IDH2 Antibody (8C3D4)

Certificate of Analysis

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Product Specific Notices for IDH2 Antibody (8C3D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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