IDH2 Antibody

Novus Biologicals | Catalog # NBP1-52117

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IDH2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CIHGLSNVKLNE corresponding to internal region according to NP_002159.2.

Epitope

CIHGLSNVKLNE

Reactivity Notes

Expected from sequence similarity: Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IDH2 Antibody

Western Blot: IDH2 Antibody [NBP1-52117]

Western Blot: IDH2 Antibody [NBP1-52117]

Western Blot: IDH2 Antibody [NBP1-52117] - 0.1ug/ml staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-52117]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-52117]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-52117] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: IDH2 Antibody [NBP1-52117]

Western Blot: IDH2 Antibody [NBP1-52117]

Western Blot: IDH2 Antibody [NBP1-52117] - Analysis of IDH2 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-52117 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IDH2 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1-0.3 ug/ml
Application Notes
Western blot: Approx 48kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9kDa according to Mouse NP_766599.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Skeletal Muscle.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Entrez Gene IDs

3418 (Human); 269951 (Mouse); 361596 (Rat)

Gene Symbol

IDH2

UniProt

NP_002159.2

Additional IDH2 Products

Product Documents for IDH2 Antibody

Certificate of Analysis

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Product Specific Notices for IDH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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