IDH2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29996

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IDH2 Primary Antibodies »

Product Specifications

Immunogen

Between 402 and 452

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IDH2 Antibody - BSA Free

IDH2 Antibody

Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]

Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]Detection of human IDH2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
IDH2 Antibody

Immunohistochemistry;IDH2 Antibody[NBP3-29975]

Immunohistochemistry;IDH2 Antibody[NBP3-29975]Detection of mouse IDH2 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000. Detection: DAB
IDH2 Antibody

Immunohistochemistry;IDH2 Antibody[NBP3-29996]

Immunohistochemistry;IDH2 Antibody[NBP3-29996]Detection of human IDH2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma and lung cancer. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000. Detection: DAB
IDH2 Antibody

Immunoprecipitation:IDH2 AntibodyNBP3-29996] -

Immunoprecipitation:IDH2 AntibodyNBP3-29996] -Detection of human IDH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody NBP3-29996 (lot NBP3-29996-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated IDH2, NBP3-29996 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for IDH2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Gene Symbol

IDH2

Additional IDH2 Products

Product Documents for IDH2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IDH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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