IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-258

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all IFN-alpha/beta R1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH. Accession # P17181

Modification

p Ser535, p Ser539

Localization

Type I membrane protein.

Specificity

Specific for IFNAR1 protein phosphorylated at Ser535,539.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]

Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]

Western Blot: IFNAR1 [p Ser535, p Ser539] Antibody [NB300-258] - Detection of Flag immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT and 3. IFNAR1 S535A and S539A mutants. Detection with NB 300-258 is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide used as antigen but not by the dephospho form of this peptide.

Applications for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Note: the molecular weight of the IFNNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130 kDa. Use in immunoprecipitation reported in scientific literature (PMID: 15337770).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFN-alpha/beta R1

Interferons are widely used therapeutic agents because of their anti-tumor and anti-viral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon Receptor(IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 288264 (Rat)

Gene Symbol

IFNAR1

UniProt

D3ZDS9 (Rat)

Additional IFN-alpha/beta R1 Products

Product Documents for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody - Azide Free

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway
Jak/STAT Signaling Pathway Thumbnail