Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody
Novus Biologicals | Catalog # NBP2-69291
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Key Product Details
Species Reactivity
Duck
Applications
Immunoelectrophoresis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
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Product Specifications
Immunogen
Purified normal Ig fractions isolated from pooled duck serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Reactivity Notes
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Specificity
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins. It does not react with any non-Ig protein in duck serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Clonality
Polyclonal
Host
Rabbit
Description
The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Applications for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody
Application
Recommended Usage
Immunoelectrophoresis
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Precipitation assays.
Precipitation assays.
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Reconstitution
Reconstitute by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2).
Formulation
Stable whole antiserum
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Product Documents for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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