Human IgD ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60058

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.024 ng/mL

Assay Range

0.024 ng/mL - 100 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human IgD. No significant cross-reactivity or interference between human IgD and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human IgD ELISA Kit (Colorimetric)

ELISA: Human IgD ELISA Kit (Colorimetric) [NBP2-60058]
Human IgD ELISA Kit (Colorimetric)

Human IgD ELISA Kit (Colorimetric) [NBP2-60058] -

Human IgD ELISA Kit (Colorimetric) [NBP2-60058] - Standard Curve Reference

Kit Contents for Human IgD ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgD

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgD present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IgD is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Immunoglobulin D

Alternate Names

Igh-5, Ighd, Immunoglobulin Delta

Gene Symbol

IGHD

Additional IgD Products

Product Documents for Human IgD ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human IgD ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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