IGFBP-1 Antibody (JE35-39)

Novus Biologicals | Catalog # NBP3-32460

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-39 expressed in HEK293
View all IGFBP-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human IGFBP-1 aa 1-259/259. (Uniprot: P08833)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGFBP-1 Antibody (JE35-39)

IGFBP-1 Antibody (JE35-39)

Western Blot: IGFBP-1 Antibody (JE35-39) [NBP3-32460]

Western Blot: IGFBP-1 Antibody (JE35-39) [NBP3-32460] - Western blot analysis of IGFBP-1 on different lysates with Rabbit anti-IGFBP-1 antibody (NBP3-32460) at 1/1,000 dilution.

Lane 1: Human placenta tissue lysate
Lane 2: Human liver tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for IGFBP-1 Antibody (JE35-39)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGFBP-1

IGFBP1 is a gene that codes for an IGF-binding protein that circulates in the plasma and prolongs the half-life of IGFs and works to either inhibit of stimulate the growth promoting effects while also promoting cell migration and has a length of 259 amino acids and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including protein-energy malnutrition, pre-eclampsia, persistent fetal circulation syndrome, Silver-Russell syndrome, growth hormone deficiency, Ehlers-Danlos syndrome, glucose intolerance, Prader-Willi syndrome, and chronic fatigue syndrome. IGFBP1 has also been shown to have interactions with IGF1, IGF2, MMP26, TF, and ARNT in pathways such as the myometrial relaxation and contraction, HIF-1-alpha transcription factor, IGF regulation, and protein metabolism pathways.

Long Name

Insulin-like Growth Factor Binding Protein 1

Alternate Names

IGFBP1

Gene Symbol

IGFBP1

Additional IGFBP-1 Products

Product Documents for IGFBP-1 Antibody (JE35-39)

Certificate of Analysis

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Product Specific Notices for IGFBP-1 Antibody (JE35-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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