IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-12364
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2 Clone # MM0342-6U23
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant IGFBP-3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2
Scientific Data Images for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
Western Blot: IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free [NBP2-12364] -
BoTA-induced endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in a thin endometrium mouse model A Venn diagram of DEGs from EtOH-treated vs. saline-treated uteri (GSE207379) and BoTA-treated vs. saline-treated (GSE146934) displaying a shared gene with a reciprocal expression; Igfbp3. B Gene–gene network analysis of DEGs of EtOH-treated vs. saline-treated uteri categorized in CC GO terms displaying a shared gene; Spp1C Immunoblotting analysis of IGFBP3 and OPN in BoTA-treated thin endometrium compared to saline-treated group. D Immunoblotting analysis of OPN in BoTA-treated thin endometrium compared to IGFBP3-treated thin endometrium. beta -actin was used as a loading control. E An experimental schedule for an application of IGFBP3 neutralizing antibody together with BoTA. F Immunoblotting analysis of IGFBP3 and OPN in response to IGFBP3 neutralizing antibody treatment. beta -actin was used as a loading control. G Immunostaining of IGFBP3 in saline-treated, BoTA-treated, and BoTA with IGFBP-3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of IGFBP3 in the whole region of uterus and the indicated region with a box is magnified in lower panel. Scale bar; 100 um. H Immunostaining of active-OPN in saline-treated, BoTA-treated, and BoTA with IGFBP3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of active-OPN in the whole region of uterus and the indicated regions (LE luminal epithelium, GE glandular epithelium) with boxes are magnified in lower panel. Scale bar: 100 um. I H&E images for saline-, BoTA-, and BoTA with IGFBP3 neutralizing antibody-treated uteri. Scale bar: 100 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36602651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free [NBP2-12364] -
BoTA-induced endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in a thin endometrium mouse model A Venn diagram of DEGs from EtOH-treated vs. saline-treated uteri (GSE207379) and BoTA-treated vs. saline-treated (GSE146934) displaying a shared gene with a reciprocal expression; Igfbp3. B Gene–gene network analysis of DEGs of EtOH-treated vs. saline-treated uteri categorized in CC GO terms displaying a shared gene; Spp1C Immunoblotting analysis of IGFBP3 and OPN in BoTA-treated thin endometrium compared to saline-treated group. D Immunoblotting analysis of OPN in BoTA-treated thin endometrium compared to IGFBP3-treated thin endometrium. beta -actin was used as a loading control. E An experimental schedule for an application of IGFBP3 neutralizing antibody together with BoTA. F Immunoblotting analysis of IGFBP3 and OPN in response to IGFBP3 neutralizing antibody treatment. beta -actin was used as a loading control. G Immunostaining of IGFBP3 in saline-treated, BoTA-treated, and BoTA with IGFBP-3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of IGFBP3 in the whole region of uterus and the indicated region with a box is magnified in lower panel. Scale bar; 100 um. H Immunostaining of active-OPN in saline-treated, BoTA-treated, and BoTA with IGFBP3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of active-OPN in the whole region of uterus and the indicated regions (LE luminal epithelium, GE glandular epithelium) with boxes are magnified in lower panel. Scale bar: 100 um. I H&E images for saline-, BoTA-, and BoTA with IGFBP3 neutralizing antibody-treated uteri. Scale bar: 100 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36602651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free [NBP2-12364] -
BoTA-induced endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in a thin endometrium mouse model A Venn diagram of DEGs from EtOH-treated vs. saline-treated uteri (GSE207379) and BoTA-treated vs. saline-treated (GSE146934) displaying a shared gene with a reciprocal expression; Igfbp3. B Gene–gene network analysis of DEGs of EtOH-treated vs. saline-treated uteri categorized in CC GO terms displaying a shared gene; Spp1C Immunoblotting analysis of IGFBP3 and OPN in BoTA-treated thin endometrium compared to saline-treated group. D Immunoblotting analysis of OPN in BoTA-treated thin endometrium compared to IGFBP3-treated thin endometrium. beta -actin was used as a loading control. E An experimental schedule for an application of IGFBP3 neutralizing antibody together with BoTA. F Immunoblotting analysis of IGFBP3 and OPN in response to IGFBP3 neutralizing antibody treatment. beta -actin was used as a loading control. G Immunostaining of IGFBP3 in saline-treated, BoTA-treated, and BoTA with IGFBP-3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of IGFBP3 in the whole region of uterus and the indicated region with a box is magnified in lower panel. Scale bar; 100 um. H Immunostaining of active-OPN in saline-treated, BoTA-treated, and BoTA with IGFBP3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of active-OPN in the whole region of uterus and the indicated regions (LE luminal epithelium, GE glandular epithelium) with boxes are magnified in lower panel. Scale bar: 100 um. I H&E images for saline-, BoTA-, and BoTA with IGFBP3 neutralizing antibody-treated uteri. Scale bar: 100 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36602651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free [NBP2-12364] -
BoTA-induced endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in a thin endometrium mouse model A Venn diagram of DEGs from EtOH-treated vs. saline-treated uteri (GSE207379) and BoTA-treated vs. saline-treated (GSE146934) displaying a shared gene with a reciprocal expression; Igfbp3. B Gene–gene network analysis of DEGs of EtOH-treated vs. saline-treated uteri categorized in CC GO terms displaying a shared gene; Spp1C Immunoblotting analysis of IGFBP3 and OPN in BoTA-treated thin endometrium compared to saline-treated group. D Immunoblotting analysis of OPN in BoTA-treated thin endometrium compared to IGFBP3-treated thin endometrium. beta -actin was used as a loading control. E An experimental schedule for an application of IGFBP3 neutralizing antibody together with BoTA. F Immunoblotting analysis of IGFBP3 and OPN in response to IGFBP3 neutralizing antibody treatment. beta -actin was used as a loading control. G Immunostaining of IGFBP3 in saline-treated, BoTA-treated, and BoTA with IGFBP-3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of IGFBP3 in the whole region of uterus and the indicated region with a box is magnified in lower panel. Scale bar; 100 um. H Immunostaining of active-OPN in saline-treated, BoTA-treated, and BoTA with IGFBP3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of active-OPN in the whole region of uterus and the indicated regions (LE luminal epithelium, GE glandular epithelium) with boxes are magnified in lower panel. Scale bar: 100 um. I H&E images for saline-, BoTA-, and BoTA with IGFBP3 neutralizing antibody-treated uteri. Scale bar: 100 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36602651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20-200
Immunohistochemistry-Paraffin
1:20-1:200
Western Blot
1:500-1:2000
Application Notes
This IGFBP3 antibody (clone MM0342-6U23) is useful for Western Blot and Immunohistochemistry-Paraffin applications. In its IHC-P validation, the antigen retrieval was performed by incubation of sections with Proteinase K (20ug/ml in PBS) for 15min at room temperature (Note: the length of PK exposure should be optimized by the user as it may depend upon the variation of fixatives and length of fixation applied to a particular specimen).
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: IGFBP-3
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
UniProt
Additional IGFBP-3 Products
Product Documents for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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