IGFBP-3 Antibody

Novus Biologicals | Catalog # NBP2-16933

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IGFBP-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGFBP-3. The exact sequence is proprietary.

Reactivity Notes

Bovine, Pig, Rhesus Monkey (80%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IGFBP-3 Antibody (NBP2-16933) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IGFBP-3 Antibody

Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933]

Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933]

Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933] - Sample: Frozen sectioned E13.5 Rat brain. Green: IGFBP3 protein stained by IGFBP3 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
IGFBP-3 Antibody

Western Blot: IGFBP-3 Antibody [NBP2-16933] -

Western Blot: IGFBP-3 Antibody [NBP2-16933] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP-3 antibody [N2C3] (NBP2-16933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
IGFBP-3 Antibody

Immunohistochemistry-Paraffin: IGFBP-3 Antibody [NBP2-16933] -

Immunohistochemistry-Paraffin: IGFBP-3 Antibody [NBP2-16933] - IGFBP-3 antibody [N2C3] detects secreted IGFBP-3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IGFBP-3 stained by IGFBP-3 antibody [N2C3] (NBP2-16933) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
IGFBP-3 Antibody

Western Blot: IGFBP-3 Antibody [NBP2-16933] -

Mouse plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody (NBP2-16933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for IGFBP-3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IGFBP-3

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Gene Symbol

IGFBP3

UniProt

P17936

Additional IGFBP-3 Products

Product Documents for IGFBP-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IGFBP-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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