Rat IgG Fc Isotype Control [FITC]

Novus Biologicals | Catalog # NBP1-97254

Novus Biologicals

Key Product Details

Species Reactivity

Rat

Applications

SDS-Page

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Rat IgG
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Product Specifications

Specificity

No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.

Host

Rat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum

Scientific Data Images for Rat IgG Fc Isotype Control [FITC]

SDS-PAGE: Rat IgG Fc Isotype Control [FITC] [NBP1-97254]

SDS-PAGE: Rat IgG Fc Isotype Control [FITC] [NBP1-97254]

SDS-Page: Rat IgG Fc Isotype Control [Fluorescein] [NBP1-97254] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment Fluorescein Conjugated. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.

Applications for Rat IgG Fc Isotype Control [FITC]

Application
Recommended Usage

SDS-Page

1:100 - 1:2000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rat IgG Fc Isotype Control [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat IgG Fc Isotype Control [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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FAQs

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