Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Rat IgG

Format

Purified
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Product Specifications

Specificity

No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.

Host

Rat

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c)

Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rat IgG Fc Isotype Control

SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]

SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]

SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-PAGE of Rat IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]

SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]

SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.

Applications for Rat IgG Fc Isotype Control

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rat IgG Fc Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat IgG Fc Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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