Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed)

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] -

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] - Effect of varied WAT masses from obese mice on vimentin & CD24 protein expression in MDA-MB-231 cells. (a) Representative images of MDA-MB-231 cells cultured with or without 200 mg or 800 mg of obese subcutaneous or visceral WAT-CM & co-stained with anti-vimentin, anti-CD24, & DAPI; scale bar = 100 µm. (b) & (c) are the relative expression of the respective markers, mean ± SEM (n = 6 biological replicates). The average protein expression from five images per replicate were normalized to DAPI & analyzed relative to the control cultures. No groups were found to be significantly different by the Friedman test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32899433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] -

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] - Mouse WAT-CM induces the expression of CD24 in mesenchymal MDA-MB-231 cells but does not affect the expression of vimentin. Representative images of MDA-MB-231 cells cultured with or without lean or obese subcutaneous or visceral WAT-CM & co-stained for vimentin, CD24, & DAPI (a); scale bar = 100 µM. (b) & (c) are the relative expression of the respective markers, mean ± SEM (n = 6 biological replicates). The average protein expression from five images per replicate were normalized to DAPI & analyzed relative to the control cultures. Significance was determined by Friedman’s test. If significant, Dunn’s test was used for further pairwise comparison; * p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32899433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] -

Immunocytochemistry/ Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] (Pre-adsorbed) [NBP1-75617] - Mouse WAT-CM induces the expression of epithelial biomarkers in mesenchymal triple negative breast cancer cell line grown in a 3D culture. (a) Representative images of MDA-MB-231 cells cultured with or without lean or obese subcutaneous or visceral WAT-CM & co-stained for E-cadherin, claudin-7, & DAPI; scale bar = 100 µm. (b) & (c) are the relative expression of the respective markers, mean ± SEM (n = 6 biological replicates). The average protein expression from five images per replicate were normalized to DAPI & analyzed relative to the control cultures. Significance was determined by the Friedman test. If significant, Dunn’s test was used for further pairwise comparison; * p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32899433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.