Human IgG1 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP3-00401

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ug/mL

Assay Range

0.31 - 20 ug/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human IgG1 in samples. No significant cross-reactivity or interference between Human IgG1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.68%

Inter-Assay Precision (Precision between assays) 4.6%

Recovery for Human IgG1 ELISA Kit (Chemiluminescence)

Recovery

86 - 106%

Linearity

Human IgG1 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human IgG1 ELISA Kit (Chemiluminescence)

Human IgG1 ELISA Kit (Chemiluminescence)

ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] -

ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Standard Curve Reference

Kit Contents for Human IgG1 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Human IgG1 ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human IgG1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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