Mouse IgG1 Isotype Control (MOPC-21)

Novus Biologicals | Catalog # NBP2-36577

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MOPC-21
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Product Specifications

Specificity

This antibody has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This Mouse IgG1 (MOPC-21) immunoglobulin is useful as an isotype control for Mouse IgG1 (MOPC-21) antibody.

Scientific Data Images for Mouse IgG1 Isotype Control (MOPC-21)

Flow Cytometry: Mouse IgG1 Isotype Control (MOPC-21) [NBP2-36577]

Flow Cytometry: Mouse IgG1 Isotype Control (MOPC-21) [NBP2-36577]

Flow Cytometry: Mouse IgG1 Isotype Control (MOPC-21) [NBP2-36577] - Analysis result with 3 human cancer lines. Image from verified customer review. Image using the FITC form of this antibody.

Applications for Mouse IgG1 Isotype Control (MOPC-21)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, approx. pH 6.5

Preservative

No Preservative

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG1

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ug/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Mouse IgG1 Isotype Control (MOPC-21)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG1 Isotype Control (MOPC-21)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Mouse IgG1 Isotype Control (MOPC-21)

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FAQs

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