IgG2 Antibody (RM118) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25975

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM118 expressed in HEK293

Format

Azide and BSA Free
View all IgG2 Primary Antibodies »

Product Specifications

Immunogen

Human IgG2

Specificity

This antibody reacts to the heavy chain of human IgG2. This antibody does not cross react to any other IgG subclasses (IgG1, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, or IgE. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IgG2 Antibody (RM118) - Azide and BSA Free (NBP2-62076) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IgG2 Antibody (RM118) - Azide and BSA Free

IgG2 Antibody (RM118) - Azide and BSA Free

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] -

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] - ELISA of human immunoglobulins shows RM118 only reacts to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2 Antibody (RM118) - Azide and BSA Free

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] -

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] - ELISA of human immunoglobulins shows RM118 only reacts to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2 Antibody (RM118) - Azide and BSA Free

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] -

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP3-25975] - A titer ELISA using RM118. The plate was coated with different amounts of human IgG2. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG2 Antibody (RM118) - Azide and BSA Free

Application
Recommended Usage

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG2

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2

Alternate Names

Constant region of heavy chain of IgG2, immunoglobulin gamma 2 (Gm marker), immunoglobulin heavy constant gamma 2 (Gm marker)

Gene Symbol

IGHG2

Additional IgG2 Products

Product Documents for IgG2 Antibody (RM118) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IgG2 Antibody (RM118) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for IgG2 Antibody (RM118) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review IgG2 Antibody (RM118) - Azide and BSA Free and earn rewards!

Have you used IgG2 Antibody (RM118) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies