IgM Antibody (1.9/3.2)

Novus Biologicals | Catalog # NBP2-61099

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat, Equine

Cited:

Rat

Applications

Validated:

ELISA, Flow Cytometry

Cited:

ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1.9/3.2
View all IgM Primary Antibodies »

Product Specifications

Immunogen

Horse immunoglobulins

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34440885)..

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Applications for IgM Antibody (1.9/3.2)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000
Application Notes
Use in ELISA reported in scientific literature (PMID:35042674)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (1.9/3.2)

Certificate of Analysis

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Product Specific Notices for IgM Antibody (1.9/3.2)

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IgM Antibody (1.9/3.2)

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Protocols

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FAQs

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