IGSF4C/SynCAM4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13192
![Western Blot: IGSF4C/SynCAM4 Antibody [NBP3-13192]](https://beta-resources.rndsystems.com/images/products/IGSF4C-SynCAM4-Antibody-Western-Blot-NBP3-13192-img0001.jpg "Western Blot: IGSF4C/SynCAM4 Antibody [NBP3-13192]")
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Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Human (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse IGSF4C/SynCAM4. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for IGSF4C/SynCAM4 Antibody - BSA Free
Western Blot: IGSF4C/SynCAM4 Antibody [NBP3-13192]
Western Blot: IGSF4C/SynCAM4 Antibody [NBP3-13192] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IGSF4C/SynCAM4 antibody (NBP3-13192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: IGSF4C/SynCAM4 Antibody [NBP3-13192]](https://beta-resources.rndsystems.com/images/products/IGSF4C-SynCAM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13192-img0003.jpg "Immunocytochemistry/ Immunofluorescence: IGSF4C/SynCAM4 Antibody [NBP3-13192]")
Immunocytochemistry/ Immunofluorescence: IGSF4C/SynCAM4 Antibody [NBP3-13192]
Immunocytochemistry/Immunofluorescence: IGSF4C/SynCAM4 Antibody [NBP3-13192] - IGSF4C/SynCAM4 antibody detects IGSF4C/SynCAM4 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGSF4C/SynCAM4 stained by IGSF4C/SynCAM4 antibody (NBP3-13192) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Frozen: IGSF4C/SynCAM4 Antibody [NBP3-13192]](https://beta-resources.rndsystems.com/images/products/IGSF4C-SynCAM4-Antibody-Immunohistochemistry-Frozen-NBP3-13192-img0002.jpg "Immunohistochemistry-Frozen: IGSF4C/SynCAM4 Antibody [NBP3-13192]")
Immunohistochemistry-Frozen: IGSF4C/SynCAM4 Antibody [NBP3-13192]
Immunohistochemistry-Frozen: IGSF4C/SynCAM4 Antibody [NBP3-13192] - IGSF4C/SynCAM4 antibody detects IGSF4C/SynCAM4 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: IGSF4C/SynCAM4 stained by IGSF4C/SynCAM4 antibody (NBP3-13192) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 minApplications for IGSF4C/SynCAM4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGSF4C/SynCAM4
Long Name
Immunoglobulin Superfamily, Member 4B
Alternate Names
CADM4, Necl-4, SynCAM4, TSLC1-like 2, TSLL2
Gene Symbol
CADM4
Additional IGSF4C/SynCAM4 Products
Product Documents for IGSF4C/SynCAM4 Antibody - BSA Free
Product Specific Notices for IGSF4C/SynCAM4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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