Key Product Details

Species Reactivity

Validated:

Mouse, Human (Negative)

Cited:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 1B1.3a

Format

BSA Free
View all IL-10 R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant extracellular region of mouse CDw210a

Reactivity Notes

Reacts with Mouse. Does not react with Human.

Specificity

NB100-63577 recognizes mouse CDw210a, a cell surface glycoprotein of approximately 110KD, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognised by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. This clone is reported to neutralize the effects of IL-10 (1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for IL-10R alpha Antibody (1B1.3a) - BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Due to low expression of, sensitive staining techniques may be required.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Entrez Gene IDs

16154 (Mouse)

Gene Symbol

IL10RA

UniProt

Q61727

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (1B1.3a) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-10R alpha Antibody (1B1.3a) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-10R alpha Antibody (1B1.3a) - BSA Free

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FAQs for IL-10R alpha Antibody (1B1.3a) - BSA Free

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  • Q: NB100-63577 we would like to know if this antibody can also be used in paraffin?

    A: Unfortunately, we have only validated our IL10RA Antibody (1B1.3a) (catalog number NB100-63577) in flow cytometry. We have not yet tried using this product for staining of tissues (either frozen or paraffin-embedded).

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail