Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
IL-18 BPc Antibody [DyLight 488]
Novus Biologicals | Catalog # AF129K
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑18 BPc
Arg25-Ser192
Accession # AAD17193
Arg25-Ser192
Accession # AAD17193
Specificity
Detects mouse IL-18 BPc in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross‑reactivity with recombinant mouse IL-18 BPd is observed and less than 1% cross-reactivity with recombinant human IL-18 BPa is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for IL-18 BPc Antibody [DyLight 488]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-18 BPc
Long Name
Interleukin 18 Binding Protein Isoform c
Alternate Names
IL18 BPc
Gene Symbol
Il18r1
Additional IL-18 BPc Products
Product Documents for IL-18 BPc Antibody [DyLight 488]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-18 BPc Antibody [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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